EzTransfectorTM Transfection Reagent
A broad spectrum,
high efficiency,
low toxicity, DNA transfection reagent
EztransfectorTM in vitro Transfection Reagent is a
cationic polymer transfect reagent which is innovated by TSZGENE,
TSZ Scientific LLC,and distributed by Biotang Inc. This cationic polymer conjugates peptides which
binding cell surface receptors. The solution is stable at 4°C. The
efficient gene transfer activity of Eztransfector is related to its
capacity for condensing DNA and binding cell surface. Eztransfector
has a high cationic-charge density potential which makes it an
excellent DNA condensing and gene-delivering agent. Eztransfector
provides superior transfection efficiency and significant lower
toxicity when compared to various cationic lipids and polymers in a
wide number of cell types.
Eztransfector TM is suitable for the transfection of with high
efficiency and minimal toxicity. Transfection can be performed in
the presence or absence of serum (Eztransfector efficiency is not
affected by the presence of serum) . Eztransfector showed
superior results when compared to many other lipid or polymer based
transfection reagents.
EztransfectorTM
outperforms FuGENE6 Reagent at a much lower cost
EztransfectorTM
Superior performance to Lipofectamine™ 2000 at a lower cost
EztransfectorTM
Superior performance to PolyFect at a lower cost
- Broad Spectrum DNA Delivery—Utilize one
transfection reagent and protocol for a variety of cells.
- Low Cellular Toxicity—Maintain cell density
and reduce experimental biases.
- High DNA Copies Transient Transfection—More DNA
copies into one cell than other reagents, ideal for Transient
Transfection.
- High Efficiency Delivery—Achieve expression
in a large population of cells for experimental success.
- Deliver Single or Multiple Plasmids—Suitable
for many applications such as gene expression, siRNA/shRNA expression,
viral production, and promoter analysis.
Cell Types Successfully Transfected
by EztransfectorTM Transfection Reagent:
--Download
cell type list here
293T, 56FHT80 Human Fetal tracheal epithelium, 6CFSMEo- Human
Submucosal gland epithelium cells, 9HTEo- Human Normal adult trachea
epithelium cells, A549 Human Type II pneumocytes, B 16F10 Mouse
Melanoma cells, BAEC, BNL CL.2 Mouse Hepatocytes, C-26 Mouse Colon
carcinoma cells, C2C12 Mouse Myoblasts, Caco-2 Human Colorectal
adenocarcinoma cells, CFNPE9o- Human Nasal epithelium cells, CFPEo-
Human Trachea epithelium cells, Corneal Epithelial cells , Corneal
Fibroblasts, Cos-7 Monkey African green monkey kidney cells, CT26
Mouse Colon carcinoma cells, DF-1, Embryonic stem cells (ES), GM2,
Hec1B cells, HCS-2/8 Human Chondrocyte-like cells, Hela cell, HeLa
Human Cervix epitheloid carcinoma cells, Hep 2C Human Epidermal
carcinoma cells, Hep G2 Human Hepatoma cells, HL-60 Human
Myelomonocytic cell line, HT 29, Human Monocytes / macrophages,
Human, Rat Postmitotic neurons, HuVEC, J-774 Mouse Myelomonocytic
cell line, Jurkat Human T cell leukemia, KB Human Epithelial cells,
KBv Human Drug-resistant derivative of KB, L929 Mouse Subcutaneous
connective tissue fi broblasts, LLC-MDR1 Porcine Drug-resistant
derivative of LLC-PK1, LLC-PK1 Porcine Kidney epithelial cells,
MCA-38 Mouse Colon carcinoma cells, MCF7 ADR Human Drug-resistant
derivative of MCF7, MCF7 Human Breast adenocarcinoma cells, Neuro2a
Mouse Neuroblastoma cells, Newborn Rats Dorsal root ganglia neurons,
NIH 3T3 Mouse Embryonic fi broblasts, Normal Human Astrocyte, Pekin
Duck, Phoenix celsl,PC-12 cell Primary cell cultures of Tupaia
belangeri Hepatocytes, SY5Y cells, THP1 Human Myelomonocytic cells,
and U87, U-937 Human Myelomonocytic cells.
*If the cells you have are not on the
list, just ask for a FREE sample to test
your cells.
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Data
Figure 1:
Figure 2:
Figure 3:
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| Figure 1. |
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| Figure 2. The EztransfectorTM
Reagent Exhibits Higher Efficiency Compared to other
Leading Transfection Reagent. |
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| Figure 3. The EztransfectorTM
Reagent Exhibits Low Cellular Toxicity Compared to
Another Leading Transfection Reagent. SY5Y human
neuronal cells were transfected using Eztransfector Reagent
or a leading competitor, Reagent L2K, according to the
manufacturer’s recommendations. The presence of cells and
the disappearance of many cells in the Reagent L2K
transfected sample, compared to the Pre-transfected cells,
illustrates the extreme cellular toxicity observed with
Reagent L2K after adding transfection reagent for 24 hours.
In contrast, the cells transfected with the Eztransfector
Reagent exhibited minimal cellular toxicity. |
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